Genetic Mapping of an Aegilops tauschii–derived Hessian Fly Resistance Gene in Common Wheat

Hessian fl y [Mayetiola destructor (Say)] is a major threat to wheat (Triticum aestivum L.) production in the eastern United States. Cultivar releases containing major Hessian fl y resistance genes have proven effective in minimizing losses caused by this pest. Nevertheless, the ephemeral nature of major gene resistance necessitates the identifi cation of novel sources of resistance. Hessian fl y resistance from Aegilops tauschii Coss. (2n = 2x = 14; genome DD) was introgressed into the genetic background of the soft red winter wheat cultivar Saluda during the development of the germplasm line NC09MDD14. Our genetic characterization and linkage mapping studies showed that resistance to Hessian Fly biotype L in NC09MDD14 was monogenic and the most likely order of the linked microsatellite markers was: Xcfd134.2 cMXcfd421.8 cMXgdm1414.9 cMXgdm361.5 cMNC09MDD14 Hf gene/Xcfd13213.4 cMXcfd19. This linkage map situated the NCD09MDD14 Hf gene on the short arm of chromosome 6D, within the same deletion bin as the named gene H13. No recombinants between H13 and the NCD09MDD14 Hf gene were found in an allelism study that included 170 F2 individuals from the cross between NCD09MDD14 and Molly (H13). The Hessian fl y resistance gene present in the germplasm line NC09MDD14 could be an allele of H13, but unlike H13, the gene in NC09MDD14 provides resistance against biotype vrH13. L.M. Miranda, USDA-ARS, 3127 Ligon St., Raleigh, NC 27607; S.E. Cambron, USDA-ARS, Crop Production and Pest Control Research Unit, West Lafayette, IN 47907; J.H. Lyerly and J.P. Murphy, Dep. of Crop Science, North Carolina State Univ., Box 7629, Raleigh, NC 27695; D.E. Bland and J. Johnson, Dep. of Crop Science, Univ. of Georgia-Griffi n Campus, 1109 Experiment St., Griffi n, GA 30223; G.D. Buntin, Dep. of Entomology, Univ. of Georgia-Griffi n Campus, 1109 Experiment St., Griffi n, GA 30223. Received 24 May 2009. *Corresponding author ([email protected]). Abbreviations: LOD, likelihood of odds; PCR, polymerase chain reaction; RFLP, restriction fragment length polymorphism; SSR, simple sequence repeat. Published in Crop Sci. 50:612–616 (2010). doi: 10.2135/cropsci2009.05.0278 Published online 22 Jan. 2010. © Crop Science Society of America | 677 S. Segoe Rd., Madison, WI 53711 USA All rights reserved. No part of this periodical may be reproduced or transmitted in any form or by any means, electronic or mechanical, including photocopying, recording, or any information storage and retrieval system, without permission in writing from the publisher. Permission for printing and for reprinting the material contained herein has been obtained by the publisher.